Year: 2022 | Month: April | Volume 12 | Issue 2
Cultural Isolation, Identification and Antibiotic Resistance of Streptococcus agalactiae from Bovine Sub-Clinical Mastitis Cases
Beenu Jain
Bharat Bhanderi
Mayurdhwaj Jhala
Anuj Tewari
DOI:10.30954/2277-940X.02.2022.8
Abstract:
Streptococcus agalactiae causes sub-clinical mastitis in cattle which subsequently causes loss of milk production and also jeopardizes the quality of milk. The aim of this study was to isolate, identify and study the antibiogram of Streptococcus agalactiae prevalent in the nearby dairy farm of Anand Agricultural University, Anand. It was found that out of 47 cows and 107 udder quarters, 39 (82.97%) cows and 82 (76.63%) udder quarters were positive for sub-clinical mastitis by California Mastitis Test (CMT). On cultural isolation from milk samples of 82 sub-clinical mastitis positive quarters, 27 Streptococci isolates were confirmed by CAMP (Christie-Atkins-Munch-Peterson) test. All the 27 Streptococci isolates were confirmed as Streptococcus agalactiae by PCR using Streptococcus agalactiae specific primers (Sag 432/ Sag 1018) for the 16S rRNA. The antibiogram pattern of the Streptococcus agalactiae isolates was studied by Disc diffusion method for the seven antibiotics. It was found that the isolates were most sensitive for Gentamycin followed by Enrofloxacin, Ampicillin, Co-trimoxazole and Erythromycin. Least sensitivity was obtained for Tetracycline and Streptomycin.
Highlights
- From 82 sub-clinical mastitis positive quarters, 27 Streptococci isolates were obtained.
- Isolates were most sensitive for gentamycin followed by Enrofloxacin, Ampicillin, Co-trimoxazole and Erythromycin. Least sensitivity was obtained for Tetracycline and Streptomycin.
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